Correlative Consecutive Sectioning with Optimized Immunoblotting
Purpose
• Enables high-sensitivity detection of proteins from very thin, precisely aligned tissue slices, which can be directly correlated with spatially resolved data from immunohistochemistry, autoradiography, or other imaging modalities.
Procedure
• One subset of tissue sections is allocated for in situ analyses such as immunohistochemistry or autoradiography, enabling the localization and quantification of specific proteins within the anatomical context. Adjacent serial sections are processed using a carefully optimized technique in which tissue is gently harvested from glass slides to obtain material suitable for quantitative immunoblotting. This allows for the precise biochemical measurement of proteins of interest, facilitating direct molecular correlation with the spatial distribution patterns observed in neighboring slices.
Applications
• Particularly useful for mapping the distribution of proteins while quantifying their total expression levels
• Linking pathology with changes in protein abundance.
• Validating imaging biomarkers
• Maximize the information obtained from precious samples.
• Correlate molecular and cellular features with spatial and biochemical precision.
