The Luminex 200 system
Why we use it
• With the Luminex xMAP technology it is possible to analyze up to 100 analytes in a single sample
Equipment
• A Bio-Plex Multiplex System, powered by the Luminex xMAP technology in combination with bead-based immunoassays
Applications in Molecular Psychiatry
• Protein profiling in blood, cerebrospinal fluid, saliva, muscles, brain and peripheral tissue from rodents, pigs, and humans.
For example, we have investigated
- Cytokines/chemokines
- Stress hormones (ACTH, melatonin, corticosterone)
- Neuropeptides
- Hormones
- Growth factors
- Phosphoproteins
Simultaneous detection of up to 100 analytes in one sample allows us to further explore the underlying molecular mechanisms associated to the pathophysiology of psychiatric disorders and to identify biomarkers.
References
Kofod J, Elfving B, Nielsen EH, Mors O, Köhler-Forsberg O. Depression and inflammation: Correlation between changes in inflammatory markers with antidepressant response and long-term prognosis. European Neuropsychopharmacology 2022 54:116-125.
Hansen SSK, Krautz R, Rago D, Havelund J, Stigliani A, Færgeman NJ, Prézelin A, Rivière J, Couturier-Tarrade A, Akimov V, Blagoev B, Elfving B, Pedersen DN, Vogel UB, Khodosevich K , Hougaard KS, Sandelin A. Pulmonary maternal immune activation does not extend through the placenta but leads to fetal metabolic adaptation. Nature Communications 2024. 15, 24 s 4711
Højgaard K, Kaadt E, Mumm BH, Pereira VS, Elfving B. Dysregulation of circadian clock gene expression patterns in a treatment-resistant animal model of depression. Journal of Neurochemistry 2024. https://doi.org/10.1111/jnc.16172.
Johannessen K, Braný D, Dvorská D, Krivošová M, Oppa M, Ondrejka I, Strnádel J, Ferencová N, Tonhajzerová I, Danková Z, Halašová E, Mokrý J, Elfving B. Dysregulated miR-1246 and miR-1253 link inflammatory protein markers in plasma to major depressive disorder in female adolescents. Brain, Behavior, and Immunity – Health 2025 49:101108. doi: 10.1016/j.bbih.2025.101108.
