The Luminex 200 system

Why we use it

• With the Luminex xMAP technology it is possible to analyze up to 100 analytes in a single sample

Equipment

• A Bio-Plex Multiplex System, powered by the Luminex xMAP technology in combination with bead-based immunoassays

Applications in Molecular Psychiatry

• Protein profiling in blood, cerebrospinal fluid, saliva, muscles, brain and peripheral tissue from rodents, pigs, and humans.
For example, we have investigated

Cytokines/chemokines
Stress hormones (ACTH, melatonin, corticosterone)
Neuropeptides
Hormones
Growth factors
Phosphoproteins

Simultaneous detection of up to 100 analytes in one sample allows us to further explore the underlying molecular mechanisms associated to the pathophysiology of psychiatric disorders and to identify biomarkers.

References

Kofod J, Elfving B, Nielsen EH, Mors O, Köhler-Forsberg O. Depression and inflammation: Correlation between changes in inflammatory markers with antidepressant response and long-term prognosis. European Neuropsychopharmacology 2022 54:116-125.
Hansen SSK, Krautz R, Rago D, Havelund J, Stigliani A_,Færgeman NJ, Prézelin A, Rivière J, Couturier-Tarrade A, Akimov V, Blagoev B, Elfving B, Pedersen DN_, Vogel UB_,Khodosevich K_,Hougaard KS, Sandelin A. Pulmonary maternal immune activation does not extend through the placenta but leads to fetal metabolic adaptation. Nature Communications 2024. 15, 24 s 4711
Højgaard K, Kaadt E, Mumm BH, Pereira VS, Elfving B. Dysregulation of circadian clock gene expression patterns in a treatment-resistant animal model of depression. Journal of Neurochemistry 2024. doi.org/10.1111/jnc.16172.