Real-time quantitative Polymerase Chain Reaction (Real-time qPCR)

Why we use it

• Real-time detection of mRNA transcripts using SybrGreen or Taqman chemistry

• Real-time detection of non-coding RNAs

Our Equipment

• Mx3005P Real-time PCR system

• ARIAMx Real-time PCR system

Applications in Molecular Psychiatry

• mRNA/non-coding RNA profiling in brain tissue and peripheral tissue from translational animal models and psychiatric patients

• RNA profiling in blood and CSF

Real-time qPCR enables us to explore specific molecular pathways to further understand pathophysiological mechanisms and novel treatment paradigms.  

References

  1. Kaadt E, Højgaard K, Mumm B, Christiansen SL, Müller HK, Damgaard CK, Elfving B. Dysregulation of miR-185, miR-193a, and miR-450a in the skin are linked to the depressive phenotype. Progress in Neuropsychopharmacology & Biological Psychiatry 2021 104:110052.
  1. Elfving B, Liebenberg N, du Jardin K, Sanchez C, Wegener G, Müller HK. Single dose S-ketamine rescues transcriptional dysregulation of Mtor and Nrp2 in the prefrontal cortex of FSL rats 1 hour but not 14 days post dosing. European Neuropsychopharmacology 2022, 65:56-67.
  1. Krivosova M, Adamcakova J, Kaadt E, Mumm BH, Dvorska D, Brany D, Dankova Z, Dohal M, Samec M, Ferencova N, Tonhajzerova I, Ondrejka I, Hrtanek I, Hutka P, Oppa M, Mokry J, Elfving B. The VEGF protein levels, miR-101-3p, and miR-122-5p are dysregulated in plasma from adolescents with major depression. Journal of Affective Disorders 2023. 334:60-68.
  1. Kaadt E, Hedemann NK, Damgaard CK, Müller HK, Elfving B. From microRNA to protein, linking the neurotrophic hypothesis of depression to the Wistar Kyoto rat. Neuroscience Applied 2023. 2:101131.
  2. Højgaard K, Kaadt E, Mumm BH, Pereira VS, Elfving B. Dysregulation of circadian clock gene expression patterns in a treatment-resistant animal model of depression. Journal of Neurochemistry 2024. doi.org/10.1111/jnc.16172.