uHPLC is an advanced form of HPLC with higher resolution, faster run times and increased sensitivity. It is used for identifying and quantifying components in complex mixtures, such as tissue, blood, cultured cells, or placental perfusates. uHPLC has many uses as it can separate and identify peptides, protein, and other biomolecules. It can also be used to analyse drug compounds and quantify the concentration of an active pharmaceutical ingredient.
• In brain, plasma and serum from humans or experimental animals we can measure neurotransmitters and their metabolites using electrochemical detection
• By application of fluorencence detection we can quantify amino acids in tissue and blood
• Very little sample is needed, making it an attractive method when analysing e.g. subregions of mouse brains
• The technique is very versatile and can be modified for many other analytes (e.g. vitamins, pharmaceuticals, hormones)
• Two ThermoScientific Ultimate 3000 systems with biocompatible isocratic pump, autosampler, and electrochemical detector
• One ThemoScientific Ultimate 3000 system with rapid separation querternary pump, autosampler, and fluoresent deterctor
• Altered expression of monoamines and monoamine degradation in different brain regions in models of psychiatric disease
• Altered expression of amino acids in blood plasma of patient populations after experimental treatments
• Fetal expression of monoamines after maternal stress as measured in ex vivo placental perfusates